WebWe will increase some of our prices on April 1, 2024. Be sure to complete your order before this time to take advantage of current prices. See the new prices and get more … WebCreative Biolabs offers high-quality rAAV-EF1α-hGtACR1-P2A-EGFP to boost neuroscience research.
Addgene th-P2A-EGFP donor - Sequence Analyzer
WebPlasmid th-P2A-EGFP donor from Dr. Jiu-lin Du's lab contains the insert th-P2A-EGFP and is published in Cell Res. 2015 Apr 7. doi: 10.1038/cr.2015.43. This plasmid is available … WebeGFP gene was amplified from th-P2A-eGFP donor with primers P2A-eGFP-F: 50-CCGGCCAGGCAAA AAAGAAAAAGGCTACTAATTTCTCCT-30 and P2A-eGFP-R: 50 … gta san andreas speedometer and gasoline mod
Precise DNA cleavage using CRISPR-SpRYgests Nature …
WebThe P2A-eGFP cassette was inserted before the stop codon to direct expression of eGFP without disrupting the endogenous Foxf1 gene, resulting in expression of separate Foxf1 and eGFP transcripts (Figures 2A and E2A–E2E). WebOct 22, 2024 · The pX330.1 plasmid was constructed as follows: The P2A-EGFP sequence was amplified by PCR from pCriMGET-3 × Flag-P2A-EGFP. Then, the fragment was inserted into the EcoRI site of pX330 ... WebOct 6, 2016 · A homologous recombination donor plasmid was designed to incorporate the P2A-EGFP cassette just upstream of the stop codon and with the puromycin selection gene downstream. FOXA2-2A-EGFP targeting donor vector, gRNA, and Cas9 expressing vectors were coelectroporated into hESCs and puromycin-resistant colonies were selected. gta san andreas speedrun world record