Web14 de out. de 2024 · In addition, luseogliflozin also inhibited the expression of hypoxia-induced hexokinase 2 (HK2) 22, which catalyzes the first step of glucose metabolism, and pyruvate kinase M1/2 (PKM) 23, a rate ... Web27 de mai. de 2014 · Osteoblast precursors residing in the bone marrow experience hypoxia, which leads to stabilization and increased activity of hypoxia-inducible factor 1α (HIF1α). However, whether an increase in HIF1α activity directly impacts bone formation is not known. Two well-known functions of HIF1α are to promote angiogenesis and to …
Effects of dapagliflozin on HIF1 in HK2 cells. (A) Representative ...
Web1 de jun. de 2024 · It has been well-established that AKT2 plays an important role in the development and progression of colon cancer; however, its precise function remains unclear. In the present study, we found that AKT2 can interact with and phosphorylate hexokinase 2 (HK2), the rate-limiting enzyme in glycolysis.Moreover, threonine … Web19 de nov. de 2024 · The ureohydrolase, type-II arginase (Arg-II), is a mitochondrial enzyme metabolizing L-arginine into urea and L-ornithine and is highly expressed in renal proximal tubular cells (PTC) and upregulated by renal ischemia. Recent studies reported contradictory results on the role of Arg-II in renal injury. The aim of our study is to … daily grind winchester ky
Up-regulation of glycolytic metabolism is required for HIF1α
Web15 de ago. de 2011 · Immunoblot analysis of these cells grown in the presence or absence of 1 μg/mL of doxycycline confirmed that HIF1α expression was induced by doxycycline and that the HIF1α levels achieved were similar to those observed after treating HK2 immortalized renal epithelial cells with the prolyl hydroxylase inhibitor … Web1 de set. de 2015 · Renal hypoxia contributes to chronic kidney disease (CKD) progression, as validated in experimental and human CKD. In the early stages, increased oxygen … All cell lines used were confirmed free from mycoplasma contamination, as determined by PCR. The human HCC cell lines HepG2 and Huh7 and lentiviral packaging line 293FT were grown in Dulbecco’s Modified Essential Media (DMEM, Hyclone), 10% fetal bovine serum (FBS, Atlanta Biologicals) or with … Ver mais Proliferation rates were assessed in cell lines by proliferation curve analysis and BrdU incorporation assays. For both assays, cells were plated on 6-cm plates in triplicate (50 × 103 … Ver mais Anchorage-independent growth assays were performed by plating 10–30 × 103cells in triplicate in 6-well plates in a mixture of media containing (0.35%) low-melt agarose over a … Ver mais To prepare samples, frozen cell pellets were lysed with lysis buffer: (20 mM) HEPES, (150 mM) NaCl, (1%) Triton X-100, (1 mM) EDTA, (1 mM) EGTA, (10 mM) sodium … Ver mais Cell death was assessed by either scoring apoptotic cells (cells with condensed or fragmented nuclei) visually at 20 × magnification after staining with the fluorescent DNA stain … Ver mais biohyace