Cisplatin h460

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WebApr 14, 2024 · To verify the resistance of A549/CR and H460/CR cells, the effect of cisplatin or PGG on viability (Figure 1A) and the PTEN expression level was examined in A549 and H460 parental cells and cisplatin-resistant A549/CR and H460/CR cells using an MTT assay and Western blotting. Cisplatin-resistant cell lines demonstrated a subtle … WebApr 9, 2024 · In vitro cytotoxicity of free cisplatin and G4.0-PAA/aquated cisplatin nano-complex against NCI H460 cells (human lung cancer cells) was evaluated by SRB assay, and their corresponding IC 50 values were calculated from the dose–response curves using non-linear regression analysis. The cells were incubated for 5 h at pH 7.4 in the …

Cisplatin-induced hydroxyl radicals mediate pro-survival …

WebResistance mechanism to cisplatin in NCI-H460 non-small cell lung cancer cell line: investigating apoptosis, autophagy, and cytogenetic damage … WebMay 27, 2024 · Epithelial non-small lung cancer H460 cells (generated from a pleural effusion of a patient with large cell lung cancer) and H1299 cells (derived from an NSCLC carcinoma) were maintained in RPMI supplemented with 10% fetal bovine serum (FBS) (Life Technologies, Grand Island, NY, USA). dark tea from china

Targeting transient receptor potential canonical 1 reduces …

WebOct 31, 2024 · Generation of Cisplatin-Resistant Lung Cancer Cell Lines A549, H460, H661, and H1299 lung cancer cells were exposed to cisplatin according to the IC50 value for 72 h. The medium was removed, and the cells were cultured in normal medium until the cells fully recovered. WebInjection, cisplatin, powder or solution, 10 mg: BETOS Code: O1D - Chemotherapy: Action Code: N - No maintenance for this code: Type of Service Code: 1 - Medical care: Pricing … WebSep 1, 2006 · Figure 2C shows that H460-pMEP 4-MKP-1 cells treated with CdSO 4 were more resistant to both doses of cisplatin compared with the control H460-pMEP 4 vector cells . Thus, these results indicate that overexpression of MKP-1 plays a critical role in cisplatin resistance in the H460 human lung cancer cell. dark tea from china crossword

SMARCA4/BRG1 Is a Novel Prognostic Biomarker Predictive of Cisplatin …

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WebApr 10, 2024 · Microarray data from resistant and sensitive cisplatin H460 lung cancer cell line: Data processing: The analysis and visualization of the microarray data was performed using Agilent Cytogenomic 3.0.1.1. Submission date: Apr 11, 2024: Last update date: Apr 10, 2024: Contact name: Carlos Rodriguez Antolin: Organization name WebJul 28, 2024 · Cisplatin-induced cytotoxicity in human lung cancer cells. a MTT assay revealed the significant reduction in the relative cell viability in lung cancer H460 cells after treatment with 10–100 µM cisplatin for 24 h. b Increased level of apoptosis was noted after culturing with cisplatin at 25–100 µM. c Bright blue fluorescence of Hoechst33342, … dark taupe corner computer desk by monarchWebC is-diamminedichloroplatinum(II) (cisplatin) is the most celebrated metal-based chemotherapeutic in the history of modern medicine.The discovery of its anticancer effects was not only opportune [1,2,3], especially during a period when cancer prevention was desperately needed, but also because medical oncology did not yet exist as a clinical … dark tattoo ideas for cover up

"WebCisplatin induces A549 and H460 cell apoptosis via the mitochondrial‑associated pathway (A and B) After treatment with 10, 20 and 40 µM cisplatin, cell apoptosis was detected using flow... " - Cisplatin h460

Cisplatin h460

Long non-coding RNA HIF1A-As2 and MYC form a double …

WebNov 5, 2024 · Oroxylin A attenuates hypoxia-induced cisplatin resistance a H460 cells were treated with cisplatin under normoxic (20% O2) or hypoxic (1% O2) condition for 36 h. The cell viability was analyzed ... WebJul 26, 2024 · Expression profiling of cisplatin resistant cells derived from H460 lung cell line. Organism: Homo sapiens: Experiment type: Expression profiling by array: …

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WebLung cancer is a malignant tumor characterized by a rapid proliferation rate, less survivability, high mortality, and metastatic potential. This review focuses on updated research about the clinical application of traditional Chinese medicine (TCM) as an adjuvant therapy to lung cancer treatment and the mechanisms of TCM effect on lung cancer in … WebApr 13, 2024 · Cisplatin‑resistant A549 (A549/CDDP) and H460 (H460/CDDP) cells were first established and were then transfected with negative control small interfering (si)RNA (si‑NC) or TRPC1 siRNA (si‑TRPC1). Cells were then treated with 740 Y‑P, a PI3K/Akt agonist. Subsequently, the sensitivity of A549/CDDP and H460/CDDP cells to CDDP …

WebMay 28, 2024 · Cells endogenously expressing high (A549 and H460 cells) versus low (EBC-1, CRL-5889 and HTB-182 cells) CDCA3 levels were untreated, cisplatin treated for 12 h (cisplatin) or cultured in... WebJun 20, 2024 · H460-Cis and A549-Cis cells were treated with 2 μ g/mL cisplatin to maintain cisplatin resistance character. All human cell lines have been authenticated using short tandem repeat (STR) profiling. Cells were maintained at 37°C in a humidified atmosphere with 5% CO 2.

Web2 days ago · LUAD cell lines H1299, H460, A549, H1975, CALU1 and CALU6, lung fibroblasts HEL299, lung bronchial epithelial cell line HBEC, normal human bronchial epithelium cell line BEAS2B, kidney embryonic ... WebJul 13, 2024 · a H460 cells were reprogrammed using the Stemgent mRNA Reprogramming System (Reprocell, Beltsville, MD, USA). Schedule for establishing R-H460 cells by transfecting the mRNAs of reprogramming...

Web检测H460细胞中p-Akt蛋白的表达。将细胞接种于6孔板中，加入不同浓度(10、20、30 μmol/L)芝麻素处理12 h后，在每孔细胞中加入100 μL的蛋白裂解液后，采用BCA试剂盒测定蛋白浓度，计算每个泳道的上样量为70 μg总蛋白，计算5× loading buffer量及超纯水量，定 …

WebFlap endonuclease 1 (FEN1) plays a crucial role in both DNA replication and damage repair. In this study, we investigated FEN1 expression and its clinical-pathological significance in non-small-cell lung cancer (NSCLC). Quantitative reverse PCR and bishop\\u0027s orchard hoursWebMay 1, 2024 · Cisplatin-resistant versions of the A549, H1299, and H460 cell lines were generated by continuous drug exposure. The long-term metabolic changes, as well as, the early response to cisplatin treatment were analyzed in both, parental and cisplatin-resistant cell lines. dark tart cherry juice for sleepWebDec 16, 2015 · H460 lung cancer cells increased cisplatin sensitivity and showed reduced repair of both . intrastrand and interstrand adducts suggesting that the mechanism of sensitivity is primarily . dark teal and gold curtainsWebApr 26, 2024 · NCI-H460/CDDP and its parental cells were plated in 96-well plates. Cells in 96-well plates were transfected with 2 ng pRL-tk (Promega) and 50 ng p53 reporter plasmid (Addgene) for 24 h with the lipofectamine 3000. Cells were treated with DMSO or BAY-598 at indicated concentrations for 24 h. dark teal aestheticWebConversely, H460 cisplatin-resistant cells showed increased tendency to develop bone metastasis. Gene expression analysis showed that several genes involved in tumor … bishop\u0027s orchardWebJul 28, 2024 · Results: Herein, role of autophagy in cisplatin resistance was indicated by a decreased cell viability and increased apoptosis in lung cancer H460 cells pre-incubated … bishop\u0027s orchardsWebApr 4, 2024 · A pilot study of 12 H460 tumor-bearing xenografts, 4 control (vehicle only) and 8 treated (combination of pemetrexed and cisplatin), revealed tumor growth inhibition of 88% in those treated with pemetrexed and cisplatin relative to vehicle only controls ( Supplemental data Figure S1 ). bishop\u0027s orchard hours